I'm trying to trim/filter low quality reads from paired-end exome-seq data, using BBDuk.
I used the command:
```
for...
33 results in 0.0030 seconds.
-
BBDuk quality filtering not producing expected result
Last edited by reliscu; 10-13-2021, 09:15 AM. -
VQSR mixture model and tranches plot doubts (GATK 4.1.7.0)
Hello everyone:
I am processing 74 germline exome sequencing samples of Mexican childhood patients on GATK 4.1.7.0. After VariantRecalibrator... -
Questions regarding human saliva gDNA extractions for NGS applications
Hi! I have some questions regarding human saliva gDNA extractions that I would hope fellow scientists that are experienced in the area could assist me.... -
Webinar: Perform large NGS assembly and analysis on any computer
Join DNASTAR Senior Product Manager Matthew Keyser to learn how to quickly assemble large NGS data sets using a simple laptop or desktop computer, including... -
Nextera DNA Exome Tagmentation Peaks
I'm new to the library prep side of sequencing and I've been having trouble getting my post-tagmentation, post-amplification libraries to match the profile... -
Next Generation Sequencing --Asking for industrial collaboration
To all vendors,
Quick Biology is asking for industrial collaborations. Our company is located in Pasadena, CA. with a focus on developing... -
Best practice for preprocessing the bam file for copy number estimation
Hi all,
There are known limitations involved in the copy number estimation from exome sequencing (Tumor/Normal).
For instance...Last edited by ty23991; 03-21-2016, 12:07 PM. -
Poor alignment rate by bowtie2 for exome sequence data
Hi
I am aligning the paired exome sequencing data, that originated from
Sorted raw data bam file -->
Converted to fastq... -
sample prep kit recommendation?
Hi I am new to NGS. and I have read a lot from your posts. thanks first.
we plan to do NGS from dsDNA for target exome sequencing. the... -
comparing exome sequencing and exome chip data
Hello everyone,
Does anyone know of a way I can compare the concordance between exome chip data and exome sequencing data for the same samples?... -
Pindel: Reference counting (Short Insertion)
I have a problem with counting sequences in reference to the Pindel software.
I am analyzing exome data (tumor vs. normal) and found a... -
Annovar annotation with refSeqSummary and refLink
Dear all,
I am currently struggling with annotation options in Annovar.
I have paired tumor normal exome sequencing data for which... -
How to analyze your own exome
Are you curiose about your own exome and genetic variants?
Would you like to get your exmoe sequences and anlyze it by yourself?
... -
Pedigree checking from exome data
Hi,
Can anyone recommend a tool for checking that samples within the same family are not mislabelled using their exome sequencing data.... -
Annotate allele frequencies in a vcf file
Hi everyone,
I want to make you a question although maybe it is really simple and easy:
I'm working with exome sequencing. How...Last edited by Elurbe; 04-03-2013, 11:07 AM.
