Hello,
I am hoping to get some help with the experimental design of my RNAseq project. We have 4 populations and 2 treatments, which in the end...
17 results in 0.0030 seconds.
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Experimental Design Violation?
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Account for confounding factor with big effect - experimental design
Hi,
I hope this is the appropriate forum for my question, please excuse me if not.
I have a question regarding my experimental... -
Pol III chip-seq experimental design
hi all,
we are planning on doing a ChIP-Seq experiment trying to identify PolIII binding sites in yeast and mouse.
As I am not really sure... -
Choosing experimental models for three-condition DE analysis
I recently got a new project to work on regarding differential expression analysis of RNA-seq data, and it's slightly different to what I've done previously... -
Individual-level RNA-Seq differential expression analysis with paired sample design
Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting... -
Individual-level RNA-Seq differential expression analysis with paired sample design
Hi all, I'd appreciate any thoughts that will help us with the experimental design for our project.
Our experimental design involves collecting... -
DESeq2: bad experimental design and "not full rank" problems
Hi all.
In my lab, we have some old RNA-seq data that were produced by other users in the past. The experimental design was completely... -
DESeq2 multiple contrasts no "control" condition
I have an odd experimental design in that I have 4 conditions A, B, C, and D (all with replicates). However there is no "control" condition... -
Experimental design to Analysis of Allele-Specific Expression poliploid no-ref genome
Hi Everybody!
I'm looking for an experimental design to perform an Analysis of Allele-Specific Expression, Hybrid Effects and Regulatory... -
Cell-type-specific RNA-Seq using genetic ablation
My lab is studying a cell population in Drosophila that is difficult to isolate, but genetically tractable. We created some flies in which we have genetically... -
Is 12.5M reads enough for transcript discovery in bacteria?
Hello SeqAnswers and thanks for your time.
I have a question about RNA-seq with sensitivity in mind. According to Genohub, 30+ M reads are...Last edited by Matt.Ralston; 03-30-2014, 12:51 PM. -
Experimental Design Given One Cell Line
We are interested in examining the effects of four different drug compounds (with 1 being a "control" in the form of a growth factor) on differential... -
Feedback on RAD-seq strategy
Hi,
I'm designing an experiment for RAD-seq and I was wondering if I could get some feedback about my experimental design.
I... -
Free app. for sample tracking and sample sheet generation
GenoLogics has officially released Clarity Run Manager, a free sample & project tracking application for Illumina HiSeq and MiSeq users. The application... -
Experimental Design: Which Kind of Replicate to Use?
We are planning to do some F2 mapping project and I'm unsure on how should I perform replicates for the parentals.
The parental strains...
