Hello Folks
We have run 24 influenza virus samples on our MiSeq and obtained 48 FASTQ files (R1 + R2 for each). We also got 24 Contig files....
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Questions about .fastq format and size
Hello,
I am working a .fastq file. I would like to use TopHat to convert .fastq to BAM files, and then convert the BAM files to count data...
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Converting RNA-Seq bam in fastq
Hi everyone!
I have to convert some RNA-Seq bam files into corrensponding paired-end fastq files.
I tried to use "samtools view"...
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fastq to bam
Hi!
Can anyone tell me is it possible to convert a .fastq file to a .bam file? How to convert or which tool can I use?
Thanks in a...
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cat fastq from different lanes ?!
Can I cat fastq files from different lanes and then aligned them ?
Example: I have 4 fastq for one sample two forward from lane 1 and two...
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How to work with local files with Crossbow ?
Dear All
I am using the version 1.2.0 of Crossbow. I have managed to get it working for a file specified in .manifest input file that points...
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Getting just a few alignments with Tophat2
I made an alignment for a 1.1GB file, the RNA-seq reads were in fastq (Illumina) format. The reference was in fasta, so i decided to convert the large...
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Tophat -j option error
Hi,
I ran tophat with the command shown below on a pair of paired-end read and obtained the bam file including the insertions, deletions,...