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FASTQ changing quality score to zero for primer sequences
I want to mask primer sequences from analysis, but I want to keep the sequences in the FASTQ to help with alignment. Searching against a primer list and... -
Need Help! Vsearch fastq_filter quality value above qmax
Hello all!
I'm new to the community and just started my first bioinformatics project. I'm filtering out merged reads with the expected... -
BAM to FASTQ picard or samtools
This post has been cross posted and can be deleted.Last edited by anoopkmr; 08-10-2018, 06:48 AM. -
ONT Data Analysis Questions
Hey Guys,
I'm collaborating with someone who has the minION. I just got my first data set, and am stoked to start looking into it! But... -
SOLID & FastQC tool kit
Hello everyone,
I have SOLiD Mate pair data which I am trying to pass through QC checks. what i did so far
1) Convert XSQ... -
troubleshoot with tophat2 or problem with my fastq files
Dear Folks,
I am using TopHat2 to map the reads, I guess, I am fulfilling all the requirements, additionally I give
/usr/local/bin/tophat2... -
SOLiD sequencing protocol and how to obtain quality of SOLiD reads?
I have just started working on NGS few weeks back and I was trying to get the pipeline for SOLiD right.
From what I understand the basic... -
i converted illumina fastq into sanger fastq, need advice
Hello dear ngs community,
I am new to this forum but allready red many threads which helped me alot.
So i found many ways in this forum to...Last edited by Aicen; 11-09-2011, 02:32 AM. -
Questions on the updated illumina quality score
The quality of my datum from an updated illumina system is sanger/illumina 1.9 which confused me very much.Could I just treated them as sanger format?...
