Hi All,
I have a question: for a relatively "good" FastQC report, do we still need to do data pre-processing?
...
91 results in 0.0035 seconds.
-
What we can learn from FastQC reports
-
Is a compositional bias in Sequence Content of initial bases in WES reads normal?
Per Base Sequence Content
Any experienced Whole Exome Sequencing analyst/experimentalist:
the Per Base Sequence Content... -
fastqc shows strange GC content
Hi,
we have a 36-samples ChIP-Seq experiment with a very strange GC-content behavior (s. images)
The samples are from yeast. This... -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached...Last edited by quokka; 07-30-2018, 06:45 PM. Reason: remove identifying information from attachments -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached... -
Opinion on FastQC output for HiSeq 4000 PE sequencing run
Hello,
I recently had four 400bp insert plant DNA libraries sequenced (2x150bp) using one HiSeq 4000 lane.
I've attached... -
FastQC results for 16s reads
Hi
I have some FastQC results I have generated from Illumina 16s sequences. I have attached an example of the results for one of the demultiplexed... -
Problem with trimming after FastQC
Hi,
After using a FastQC I noticed that I have red flags in Per base sequence content and in Per base content.
I used Trimmomatic... -
low GC% peak in one end of paired end reads
Hi,
I have paired end RNA seq data prepared from Brassica napus using TruSeq kit. After adapter trimming, FastQC shows a second low GC% peak per... -
Fastqc
Dear members.
I am using FASTQC* for the first time (in Galaxy).
Is there an online resource showing how to deal with a range of RNAseq... -
FastQC, v0.11.5, error: Too many tiles ...
Ubuntu
Hi,
I have FastQC, v0.11.5 and I want to evaluate an Illumina run (pairend). I have reads of 150bp and a average... -
5' Kmers problem with whole genome re-sequencing data
Hi all,
I am having some issues with getting rid of kmers in my sequencing data sets. We have re-sequenced several African buffalo genomes... -
Understanding FastQC output before Vs after trim_galore
I am very new to genome assembly and teaching myself about pre-assembly QC steps.
I performed fastQC analyses on HiSeq4000 data for forward... -
Variable per tile sequence quality across amplicon-seq fastqs
I sequenced numerous amplicon-seq libraries today and found the sequence per tile quality to be highly variable across the fastq files. I initially thought... -
Fastqc Per sequence quality scores - Two peaks?
Hi,
I am struggling to find possible explanations for seeing two distinct peaks in my "per sequence quality scores". I am very...
