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Illumina HiSeq2500 Pipeline Question
Hi there,
I have just received my Illumina HiSeq data back. I have 2x250bp runs (so forward and reverse reads).
I ran a FASTQC and...
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flash histogram output
Hello all. in the output after running flash there is a histogram labeled out.histogram which gives a plot and out.hist which gives values that can be...
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Soap denovo input files in contig file from FLASH
I am using Soap denovo for whole genome assembly. I have use trimmonatic and Flash to trim and merge my reads. Flash spits out 4 output files. My question...
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Introducing BBMerge: A paired-end read merger
I'd like to introduce a new read-pairing tool, BBMerge, and present a comparison with some similar tools, FLASH, PEAR, COPE, and fastq-join. BBMerge...
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FLASH not pairing obvious reads
I've been trying to use FLASH to combine reads that should overlap in read 1 and 2 in a recent MiSeq 2x251 run, but it seems to be missing obvious overlaps....
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FLASH : command not found
Hi there,
I'm a newbie in bioinformatics and I encounter a simple problem with FLASH (Fast Length Adjustment for SHort reads).
I'm working...