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How many lanes do I need?
I will be performing an RNA-seq experiment for differential gene expression with Illumina HiSeq 2500 (100 or 150bp pair-end). My experiment is designed... -
Miniseq problem help
An assay that I have run on the miniseq previously is starting to give me problems and I am at a loss on what it could be. My problem on the surface looks... -
MiSeq Flow Cell Tile size
Hello,
We are using MiSeq v3 for our sequencing application and I have some questions about the MiSeq Flow cell.
- The distribution
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pooling libraries across multiple lanes
Dear SEQanswers experts - I have a set of 24 samples for RNAseq. The analyses I need to do is pairwise (i.e. 12 pairs of samples) for differential gene... -
Illumina flowcell to fastq
I'm a bit unsure about the process of getting a fastq-file from an Illumina flowcell. Do they obtain a fastq-file per lane, are there multiple files per... -
Re-use empty lanes
I found out today that AB have a protocol for re-using empty flowcell lanes. I was told they aren't distributing it but will hand it out if requested.... -
Illumina Flowcells
Are there different flowcells for Paired End or single read sequencing chemistry or are the flowcells the same - just use different sequencing chemis... -
binning Illumina reads by clusters on flowcell
Just wondering is it possible to group the reads by the cluster they
came from on the flowcell. We want to do so to get an idea of regions(clusters)... -
FRT-seq: amplification-free, strand-specific transcriptome sequencing
Brief Communication
Nature Methods 7, 130 - 132 (2010)
Published online: 17 January 2010 | doi:10.1038/nmeth.1417
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Is the flowcell's layout known to affect perceived cluster density on a given tile?
I just did my first sequencing run on the GAIIx. I was doing some quality control calculations from the data contained in the Summary File and I noticed...
