A beginner user of sequencing work here.
I am going to run 100 samples on Miseq to look at the soil microbial communities (for bacteria, archaea...
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Sanger Sequencing for Community Analysis
Hello
I have environmental cDNA left over from a tricky qPCR project. Is it possible to use Sanger Sequencing of the ITS 5.8S rDNA region to observe...
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Pairwise sequence comparison tools for larger sequence
I want to compare some nucleotide sequences coding to protein of Beauveria bassiana & Metarhizium anisopliae (Entomopathogenic fungi). Please suggest...
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koushik started a topic Pairwise comparison of genes which are common between two different species in fungiin GeneralPairwise comparison of genes which are common between two different species in fungi
After completing the genome annotation of two different entomopathogenic fungi species namely Beauveria bassiana and Metarhizium anisopliae. How can we...
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common mRNA isolation kit for bacteria and fungi
Dear Folks,
Just curious to know about any mRNA isolation protocol available today that works for both bacterial and fungi. I have an NGS experiment...
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biom from QIIME to MEGAN
Greetings all,
I was wondering if someone could help me with an issue importing a .biom file from QIIME into MEGAN. All works well when...
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Advice for de novo sequencing strategy
Hi
We are considering to do a genome sequencing in one of our filamentous fungi, and would like to have some advices and hear your experiences...
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Fungal genome annotation
Hi,
I have sequenced the genome of a fungus with very little information, no EST or protein are aviable.
For the gene prediction...
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Eukaryote ARB Database
Hi all,
I am doing some studies looking at microbial diversity and I am getting a significant level of eukaryotes (mostly fungi). I do...