Location: Institute of Human Genetics, University Clinics Leipzig
and Pediatric Research Center, University Hospital for Children and Adolescents,...
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PhD Position transcriptomics and molecular genetics
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Sequins: spike-in standards for RNA sequencing
RNA sequencing (RNA-seq) has become a central tool with wide-ranging research and clinical applications. However, accurate resolution of gene expression... -
trim_galore - when to stop trimming?
I've just used trim_galore (arguments below) to trim the adapter sequences off of fastq files from Illumina hiseq 4000 run TruSeq prep. Cutadapt seemed... -
DESeq2 for targeted RNA-Seq
Hi,
We plan to run some targeted whole transcriptome rna-seq using the AmpliSeq RNA methodology currently being sold by Life Technologies/Thermo... -
Are unequal pool sizes for RNA-seq acceptable?
Background:
I want to find differentially expressed genes between wild type (WT) and knockout (KO) mice of mixed background using RNA-seq (Illumina... -
Gene expression analysis-comparsion
I have normal tissue (control A, control B) and abnormal tissue (treatment A, treatment B). I need to do differential gene expression analysis. How can... -
cufflinks's gene model options
Hi all,
I am going to use cufflinks for gene exprssion profiling in my RNA-seq analysis.
There are two way to work gene expression...Last edited by dolsemtl; 02-14-2013, 10:28 PM. -
Help Using DESeq without replicates
Hello,
I'm working with a data set that we have yet to replicate. I'm trying to take a cursory look at the differential expression, but... -
De novo assembly of nanoCAGE sequences
I am considering nanoCAGE (http://www.ncbi.nlm.nih.gov/pubmed/20543846) for non-model plant species (no or only poorly annotated genomes available) for... -
Bioinformatics Postdoctoral Fellow in Neuroscience Lab - Rockefeller University
Description:
The Greengard laboratory (Rockefeller University, New York, USA) is accepting applications from outstanding individuals for... -
Identifying differentially expressed genes in breast cancer expression profiles.
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