Hi
im trying to make hybrid assembly for Ecoli using MiSeq and Nanopore data in Masurca. currently i m getting two errors marked in Bold...
18 results in 0.0029 seconds.
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errors at the beginning of hybrid assembly using MaSuRCA-4.0.3
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Spades: Failed to determine erroneous kmer threshold. Threshold set to: 22
Hi,
Thank you for developing SPAdes for hybrid assembly.
I tried to assemble Illumina pair-end with Oxford Nanopore Sequence for... -
SPADES: Failed to determine erroneous kmer threshold. Threshold set to: 22
Hi,
Thank you for developing SPAdes for hybrid assembly.
I tried to assemble Illumina pair-end with Oxford Nanopore Sequence for... -
Combining PacBio assembly with 10X assembly?
Dear all,
Currently we created an assembly of a highly repetitive plant genome with
60x coverage of Pacbio reads (assembler HGAP4).
... -
Should I try hybrid assembly with my PacBio data?
Hi all,
I recently had the genome of a bacterial strain I am working with sequenced using both PacBio and Illumina paired end.
... -
Hybrid assembly using HiSeq and MiSeq data
I'm trying to assemble a relatively large insect genome (~ 1.5 Gbp) and have sequencing data from two different sequencing platforms that I want to combine,... -
Hybrid self-contaminants in transcriptome
Hello
I`m looking for a software, which allows to find contigs created from two or more gene fragments, split them into adequate fragments... -
illumina 454 de novo hybrid
Hi all,
I am currently trying to assemble a 5mb bacterial genome. I have 43bp single end reads from an Illumina Genome Analyzer IIx and 500bp...Last edited by pmart1; 06-30-2014, 01:35 PM. -
Hybrid assembling using PacBioToCA?
This may be a silly question. I know PacBioToCA can be used to correct PacBio long reads by alignment from, for example, Illumina short reads. Then the... -
Do I have to install velvet twice to run color and base space?
Hello, I want to install velvet to run sometimes sequence space (454), some times color space (SOLiD), and sometimes both.I see that in the installation... -
Problem with hybrid assebly with Celera Assembly
Hello, everyone,
Celera Assembler(CA) 6.1 can do hybrid assembly now.
I have 3 types of reads, Solexa (100bp read length, 500bp... -
Hybrid assembly of PacBio and Illumina
I would like to know how people are handling hybrid assemblies of PacBio and Illumina. Are Velvet or the CLC-Bio good choices?
Thanks for... -
MIRA transcriptome assembly and isoforms
Perhaps, just a quick question. I am in the process of figuring out what type of sequencing runs to do for a de novo transcriptome assembly effort for... -
Using PHRAP to assemble 454 contigs and Sanger reads
Hi all,
I'm a beginner in sequence assembly and have a question about using the assembler, PHRAP.
I'm trying to use PHRAP... -
Denovo Hybrid Assembly using 454/illumina
Hi,
I have managed to get a reasonable assembly of a bacterial genome using newbler based on 454 NG sequence data.
I have...
