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Can I use secondary antibody as my negative IP control in my ChIP seq experiment?
I am doing a ChIP experiment for H3K9me3 marks in HEK293T cells. We have the ChIP grade Ab for H3K9me3 but didn't order ab for isotype control. We have...
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No DNA in ChIP IgG Control - Strange?
Hi Guys,
Thanks in advance for your help!
Background:
It's my first time doing ChIP. I am testing a native ChIP protocol...