Hi!
I am from India and new to this forum.
I have no experience but still want to study differential mRNA and microRNA...
17 results in 0.0022 seconds.
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Strategy and Kits for mRNA and microRNA information from same sequencing
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ChIPSeq sequencing depth for histone modifications
Hi,
We would like to sequence mouse Histone modification (H3K4me3,H3K4me1,H3K27me3,H3K27ac) chip-seq samples using illumina Hiseq2500. Previously... -
Base distribution plot split issue
Hi, all. I am a freshman using illumina machines.
We have a Hiseq2500 and did PE125 runs. But the results to me are curious.
Please see...Last edited by acdan; 11-18-2015, 05:24 PM. -
Illumina HiSeq k-mers
Hi
This is about the Illumina Hiseq based on Trueseq library.
Prior to trimming, the fastqc showed some k-mers enrichment in the... -
metatranscriptomics protocol
Hi all,
we're planning to do some metagenomics and metatranscriptomics runs of 5 sediment samples along a depth gradient in a lake,...Last edited by furor; 03-21-2015, 09:29 AM. -
Cufflinks - top 100 genes
Dear All,
I'm new to RNA-Seq and Bioinformatics. We have done a RNA-Seq study using Illumina Hi-Seq (paired-end, 90bp reads) on two cell... -
Feedback on RAD-seq strategy
Hi,
I'm designing an experiment for RAD-seq and I was wondering if I could get some feedback about my experimental design.
I... -
which denovo assemble to use for assembly of meta-transcriptome data
Hi,
I have couple of questions.
Firstly, I am looking for a denovo assembly tool that can assemble a meta-transcriptome... -
Optimal coverage for RNA-seq analysis of DGE in bacteria
Hi all,
I am planning an experiment to look at DGE in a bacterial culture before and after nutrient depletion. We will be doing RNA-seq using...Last edited by seb330; 11-13-2012, 07:06 PM. -
$1,000 Exomes|$6,500 Genomes from EdgeBio
While you're waiting for the $1,000 Genome - get a $1,000 exome! Or a $6,500 Whole Genome. Just submit a minimum of 6 exomes or 3 whole genomes and we'll... -
NaN on fitNbinomGLMs using DESeq
Hi,
I am doing some RNASeq analysis and, for some of the fitNbinomGLMs I get NaN scores on some of the factors
Any idea on why this... -
RNAseq and Differential Expression
Dear all,
I have to find the best way to measure the differential expression in samples subjected to RNA sequencing.
I briefly summary the... -
After doing Bowtie2 alignment I ended up in less data.
I am having single end sequence data from Illumina and did the mapping using the Bowtie2 default parameters. Then I converted it to bam format from sam... -
pre-small RNA library prep
I am attempting to prep a small rna library and have heard of people selecting for the small rna prior to introduction into protocols. I was wondering... -
Sequence transposon flanking region
I am new to NGS first of all. I would like to sequence transposon flanking region, thus during PCR amplification step, instead of using primers supplied...
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