I'm new here, and I'm in the process of preparing a PCR-generated amplicon sequencing library (composed of many samples) that will...
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Illumina (NovaSeq 6000) cluster registration?
Hi folks,
I'm new here, and I'm in the process of preparing a PCR-generated amplicon sequencing library (composed of many samples) that will... -
My Unique Dual Indices with Nextera adapters, dont bind with any library preparation method
Hello everyone!
We designed Unique Dual Indices, with both nextera adapter sequences and truseq so we can see which performs better, in the... -
HiSeq X run: i5 index is reverse complement of i7??
Hello, I'm puzzled over an issue we are having with an Illumina HiSeq x run we completed recently for a number of reasons.
We were following... -
If you use IEM, watch out for A027
We just completed a sequencing run that used the Illumina A027 (or the AR027) index on one of the samples. Setting it up on the NEWER version of IEM incorrectly... -
index contamination in multiplexed run
Hi all,
I have an issue with adapter/index contamination in a multiplexed lane:
I multiplexed 8 samples in a single HiSeq... -
Index sequences
Hi All
I recently got RNA seq data sequenced on Illumina, which is of single sample run on single lane. When I checked the RNA seq reads...
