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Hi All
I recently got RNA seq data sequenced on Illumina, which is of single sample run on single lane. When I checked the RNA seq reads which are 94 bp in length, in RNA seq header I find that it contains 6 types of index sequences and one of the index sequence is 94% of total reads and other reads contains 5 types of different index sequences.
My question is that, is it possible that you run the same sample with different types of index sequences? But does it make sense.
I checked in the other sequences reads (5%) by mapping the reads to a reference genome, I find those reads coming from the sample which was send for sequencing from the same sample. So why the company ran the sample with so many index sequences. Can some one help me understand this question.
Best Regards
I recently got RNA seq data sequenced on Illumina, which is of single sample run on single lane. When I checked the RNA seq reads which are 94 bp in length, in RNA seq header I find that it contains 6 types of index sequences and one of the index sequence is 94% of total reads and other reads contains 5 types of different index sequences.
My question is that, is it possible that you run the same sample with different types of index sequences? But does it make sense.
I checked in the other sequences reads (5%) by mapping the reads to a reference genome, I find those reads coming from the sample which was send for sequencing from the same sample. So why the company ran the sample with so many index sequences. Can some one help me understand this question.
Best Regards
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