Has anybody got any experience in pooling 1000 barcodes?
I am planning a nested multiplex PCR, testing several loci in 1000 clonal colonies for...
8 results in 0.0022 seconds.
-
1000 barcodes
-
Synthesis of i5 index
Hi everyone,
i am new to the field and I have a problem of running out of the E505 and E506 index (of the Nextera DNA Exome (formerly TruSeq Rapid... -
MiSeq indexing with Custom Select Oligos
Hello,
We are using the Nextera Rapid Capture Enrichment kit with Custom Select Oligos to index and equimolar pool 24 samples for a 2x300 PE run... -
Tophat2 and hg19 - fail to generate fasta from indexes
Hi!
I just wanted to do a test run with Tophat2 but it appears more difficult than anticipated...
So I installed SAMtools and Bowtie2... -
Lots of "N" in the barcode.
Dear All
I am new to NGS and GAII.
After several test running ,we have a big problem
.
We usually... -
Barcodes & biases
Hi there,
Has anyone observed barcode-specific biases in his/her NGS samples?
I am aware of the miRNA-seq papers on that... -
RTA v2.8 : Conflicts with low complexity sequence
We routinely use an in-line barcoding system with bases 1-4 as variable barcode nucleotides and base 5 is a mandatory T in all indexed libraries.
... -
Building bfast index with btestindexes
The command "bfast index" requires the three parameters -f (input file), -m (mask or spaced seed), and -w (hash width or index's index). For...Last edited by bre; 01-05-2010, 01:24 PM.
