Hi,
We are about to start a fairly large experiment with the Nextera Flex kit in a very limited time. After some testing and reading experiences...
12 results in 0.0022 seconds.
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1/5 Nextera Flex number of cycles
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Excessive adapter dimer in Chip input samples but not in IP samples
Hello all. I have been coming across an issue during my chip seq library prep that has persisted for months now. I am preparing ChiP seq libraries using... -
Agilent SureSelectXT - input
Hello,
I have been using the recommended input of 3ug for Aglient SureSelectXT but when I QC post covaris/post clean-up, I am left with...Last edited by sward; 10-21-2016, 03:24 AM. -
Treatment of ChIP-Seq Input
Hello all! I have placed this in the Bioinformatics forum because I believe this might be a more statistical problem, but if I'm incorrect, please don't... -
whether should keep same number of mate reads as input into Tophat
Hello all,
I use fastx-toolkit to fliter low quality reads from paired-end reads files respectively, which will lead to the different amount... -
How many inputs for each replicate/cell line
I have some samples ready to sequence but I having some doubts about the number of inputs to sequence.
The samples come from 2 human cell... -
ChIP-seq Library Prep/Multiplexing?
Hello everyone,
This is my very first post to the site, please be gentle! I have read a lot of good ChIP-seq papers, and I have scoured... -
non tagged strain biases with HA antibodies
Hello,
I'm currently encountering a problem with my ChIP-seq analysis, for some highly expressed genes I have a peak on the ORF in the non tagged... -
Bowtie paired read input format
Hi there, I have some fastq data that is formatted for use with Trinity, which supports unpaired reads in paired end data (that is, some sequences that... -
SVA input files
Hi,
I have only VCF and GVF with me. How do I convert these files to SVA compatible input files like samtools? Can i load VCF directly into ... -
Can I randomly sampe from ChIP seq input
I have a problem for peak calling. Could anyone help me?
After bowtie, I am using MACS to call peaks. There are 12 million unique reads in input,... -
Library input on the cluster station GA2
Hi!
Does anyone know the optimal input on the cluster station for GDNA PE Seq?
The protocol recommends 1-4 picoMolar (pM) but we...
