34 results in 0.0030 seconds.
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Weird distribution of insert size
I got a strange distribution of insert size as analyzed by Qualimap on the BAM file. Not totally aware of the library preparation procedure yet, but this... -
HiSeq X different insert size?
I am about to prepare TruSeq PCR-free libraries for HiSeq X sequencing.
From:
http://support.illumina.com/sequenci...uencing_instru... -
bowtie2 expected insert size interval
Hey everyone,
I'm in a bit of a pickle trying to figure out the way the output in bowtie2 is different from the bowtie'1' output. My... -
illumina aligment ratio reduced when increase insert size
We build two different cDNA library for the same sample with different size(196 and 225) for pair end sequencing by Hiseq with the same sequence length... -
tophat2 aligment ratio reduced when increase insert size
Now I have two set of HiSeq pair end equencing data for the same sample with different insert size(196 and 225). The I use tophat2 to align the reads... -
Nextera XT w/ metagenomes of various sizes
I'm planning to make libraries from several metagenomic DNA samples using Nextera XT for the purpose of de novo assembly of microbial genomes. I'm unconcerned... -
Problem with the insert size of RNA-seq paired end reads!
The problem is that I cannot get a huge part of my RNA-seq data aligned!
These are some samples from the liver, intestine and colon of mice. We...Last edited by rozitaa; 02-24-2015, 05:21 AM. -
How to correctly estimate RNA-seq mean insert size and standard distribution
Hi SEQanswers community!
So I'm not sure if this has been posted - I poked around, but didn't find anything specific enough so I'll just... -
Skewness in insert size of RNA-Seq data
Hi,
I aligned Illumina fastq reads (2x100bp) to transcriptome using Bowtie2 and found that the mean insert size is ~180bp and SD ~60bp.... -
Sawtooth base frequency, wavy insert size histograms.
I am analyzing some NextSeq data and see odd patterns in the insert size and base composition histograms, that I can't explain. The library is of a bacteria... -
Estimated very low inset size from bam file using picard
Hi,
I have used picard's CollectInsertSizeMetrics program to collect insert size matrices from bam file. Although program worked quite... -
Calculating coverage based on full insert size from paired-end data
Hi! all,
I would like to calculate coverage from paired-end data. I can do this with bedtools but I want to make sure of one thing. That... -
Uploading MiSeq mate-pair in CLC Genomics Workbench
Dear all,
I'm trying to import MiSeq FastQ files to CLC Genomics Workbench (version 4 point something), and I want to make sure that I'm... -
Truseq: insert size <100bp complications
Hello community,
I currently have a designed custom amplicon panel with insert sizes around 100bp which for the most part work fine. I'm noticing... -
Naive question about Cuffdiff log
I have been looking at posts about following question and could not find a convincing answer.
In the logs generated by cuffdiff , we saw...
