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Finding T-DNA insertion events in complex genome
Hi, I need to design a sequencing experiment to locate about a dozen insertion events of a ~10kbp transgene of known sequence in a very complex plant...Last edited by flef; 02-24-2016, 04:57 PM.
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Expected insertion: deletion ratio for exome data?
Hi Everyone,
I am wondering what the expected insertion: deletion ratio is for your exomes? Right now I'm getting ~0.9 meaning deletions...
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Ion Torrent data quality impressions?
Hi.
We have an Ion Torrent PGM where we have sequenced a number of genomes. Looking at the TMAP alignments we definitely see sequencing errors...