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Ion Proton S5 Chips - 540
Hello all, I am interested in purchasing the new Ion instrument, the S5 Prime, and was wondering if anyone has worked with the 540 chips before. We previously... -
Difference between Ion torrent proton and Ion PGM
Dear all,
I am completely new to ion proton data. I've been reading papers that were discussing about Ion torrent (most of the times I... -
Problem generating library for miRNAs from plasma
Hi all,
We've been having a hard time generating a library from plasma miRNA to use with Ion Proton. So far we've tried Life Tech's RNAseq... -
has anyone used a hotspot file yet?
Hi everyone,
I was just wondering if anyone here has ever used a hotspot vcf in order to obtain homozygous reference genotypes.
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Ion Proton for allele-specific ChIP-seq, RNA-seq
Hi,
In our lab we are working with allele-specific ChIP-seqs and RNA-seqs. After standard ChIP-seq or RNA-seq protocol we attribute obtained... -
ion proton. Reads length much shorter than library size?
Hi,
Recently I'm doing RNAseq on ion proton. I used ion total-RNA seq v2 kit to make the libraries and checked the size distribution on... -
comparison of Ion Proton RNAseq data with Illumina pair end data?
Hello everyone,
Can we compare Ion Proton RNAseq data with Illumina paired end data for expression analysis?
Thank you in... -
chimeric RNAseq reads on Ion Proton??
Hi All,
I am noticing evidence of chimeric reads coming from the ion proton, ie, the reads are half my organism of interest (lets say human) and... -
RNase III digestion time for Ion Total RNA-seq
We are doing poly A selection from 100ng of Total RNA. After building the libraries, the insert size looks to be around 100bp. We are wondering if the... -
Look for somatic mutation from Ion Proton Exome Sequencing
I've used Strelka and MuTect to find somatic mutations in SOLiD and Illumina data. They worked pretty well, but not so well for Ion Proton Exome (yes,...
