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Compression of Ion Torrent BAM files
I am happy to announce that my Genozip tool now has an optimization feature specifically for Ion Torrent BAM files: --optimize-ZM optimizes the ZM field...Last edited by divon; 08-15-2021, 05:14 PM.
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Compressing Ion Torrent BAM files
Hi all,
I just thought it might be useful to announce here that my Genozip tool can very effectively compress IonTorrent BAM files with...
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Ion Torrent Platform Differences
Hello all,
New to the forum and to the Ion Torrent platform.
From reading the literature I have noticed various different...
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Tools for Ion Torrent RNA-Seq data analysis
Hello Everyone,
I'm aware about the tools that need to be used for RNA-Seq data analysis of illumina platform.
But I would...
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Mate pair de novo assembly
Hi everyone, i am having some problems with a de novo assembly. I have two MatePair files, one from Illumina MiSeQ and the other from IonTorrent. My problem...
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jennifer_jy started a topic What is the material and size of micro-bead used in ION Torrent 314-318 chipin Ion TorrentWhat is the material and size of micro-bead used in ION Torrent 314-318 chip
Our Lab decided to purchase micro-bead for ION Torrent 314,316,318 chip, but it's hard to find the detail information of the size and material of the...
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Design of barcoding primers for ion torrent NGS of a CRISPR genome wide screen
Hello everybody,
We are going to make a CRISPR based genome wide screen as described by shalem et al (Science 2014). However, we are going...
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Best Assembler for Metagenome Assembly
Hii All,
I have couple of questions regarding metagenomic assembly..
1. What is the best assembler (open source please!!)...
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Clustering a two-D matrix
Hii All,
I have generated a 2-D matrix based on NGS data which has cog scores in one axis (Y-axis) and sample no in other axis. Now i want...
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Too many DEG with edgeR output?
Hi there,
I'm dealing with bacterial RNA-seq analysis. My experiment is very simple. Two samples to compare and no replicates. Reads were...
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Ion torrent PCR duplicates
Hi All,
I am looking at some targeted ion torrent sequencing data. Over 90% of mapped reads are PCR duplicates. Is this normal? I thought...
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extract subset of fastq based on length sequence??
Hi,
Does anyone has a script in Perl to extract a subset of fastq sequences based on length sequence?
thanks very much!...
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Bioinformatics
Hi All, I am new to this forum. Hoping to gain lots of information from you guys. Looking forward to your inputs on Iontorrent-based sequencing data....
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Lots of chimeric transcripts Ion PGM®
Hello Bioinformatic community,
We have recently sequenced a bacterial transcriptome with 316 chip from IonTorrent (1.5 million sequences)....
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==> Argon consumption
Any experience how many cubic feet of Argon are used for a certain number of runs?
It's also not clear to me what the specs for the Argon...