Hello,
I have been using the Kapa Hyper Prep kit to try and prep DNA libraries for target enrichment.
I am using custom Y adapters and...
12 results in 0.0023 seconds.
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No amplification with Kapa kit
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RNA-Seq on FFPE samples : Illumina TruSeq RNA exome or Roch Kapa HMR kit
Hi,
I've ~300 FFPE samples to analyzed using RNA-Seq. I've two possibilities for the library prep depending on the sequencing center I... -
Illumina library quantification using a QuantStudio 5
Hello,
Does anyone have any successful experience using a QuantStudio 5 to quantify their illumina libraries? I've been trying to get decent... -
KAPA index adapter compatibility
Hello,
Has anyone used the KAPA single-indexed adapter kit with a library prep kit other than one of KAPA's? I would like to test out their newer... -
qPCR issues
Hello, we use Kapa qPCR kits to quantify our libraries before sequencing. Recently, the NTC wells are giving low cq values which means there are contaminants... -
Kapa vs NEBnext for ChIPSeq?
Hi Seqanswers!
Forgive me if there’s already a post on this (I saw a post comparing Truseq ChIP kit with NEBnext, but nothing on Kapa) but does... -
RNAseq - Alternatives to SeqScript?
Howdy all,
Looking for lower cost alternatives to SeqScript. I realize a big portion of the cost is tied up in the Ribo Zero depletion... -
Automated Library Prep Instruments
I'm looking for input on Wafergen's Apollo 324 and Perkin Elmer's NGS Express.
If you have (or have used) one, do you like it? Are there... -
KAPA qPCR troubleshooting
Hi,
I'm having difficulties with using qPCR to quantify my PCR-free libraries. There is just too much inconsistency in the end concentrations... -
ChIP-Seq library prep kit, Illumina vs Kapa
We are sending ChIP samples for sequencing on a HiSeq 2500. We sent some to a facility a while ago and they used an Illumina kit for library prep. Now... -
qPCR settings
Hello,
What threshold do you set for a qPCR run for quantifying libraries? We use KAPA Library Quantification Kits on an Eco instrument. We currently... -
Library Quantification Confusion!
I have just finished making some Illumina mRNA libraries using the v2 TruSeq Kit. I used the QUBIT to quantify samples, and got high concentrations (200-600ng/uL)....
