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Ichisen · 03-21-2020, 06:13 PM

I'm extremely new to this and I apologize if this question seems naive. I created a database for virulence factors and did BLASTP.

I got...

trauba · 05-05-2016, 06:51 AM

What exactly is it that limits the read length anyways?

Also, eventually some oligos get "out of phase" and then muddy the signal....

TristanB · 04-09-2016, 11:27 AM

Hi all,

I would like to compare average fragment length among reads contributing to a minor allele at a given heterozygous position vs...

ebe · 07-02-2014, 12:52 AM

Hi,

we sequence human DNA with 454 Junior, single-read. Runs are ok (number and quality of reads).
After trimming (quality>20 or...

fibar · 07-15-2013, 07:16 AM

Our goal: to reconstruct individual genomes from a metagenome
Our method: Illumina Hiseq paired-end sequencing

What would you choose...

dapizarro · 05-24-2013, 03:41 AM

Hi,

Does anyone has a script in Perl to extract a subset of fastq sequences based on length sequence?

thanks very much!...

zarthon59 · 11-10-2012, 09:14 AM

Hello everybody,

I was wondering if it was possible, from a list of its isoform lengths to calculate the gene length. I know RSEM is doing...

Hkins552 · 07-05-2011, 07:16 AM

Hi All,
I have GATK error when inputing the following:

$ java -jar GenomeAnalysisTK.jar -I /input.bam -R /hg19.fasta
-B:dbSNP,VCF...

chkuo · 06-02-2011, 09:49 AM

Hi all,

We are using Illumina 150bp paired-end reads to perform de novo assembly for a bacterial genome (~5Mb). Our procedure goes like...

DNAjunk · 01-12-2011, 08:01 AM

Hello

I have got a question related to the ma2bam conversion.

Is it somehow possible the output the raw read sequence and...

DNAjunk · 08-20-2009, 07:49 AM

Hi

I've made assemblies of several 454 reads (in sff and fasta format) by using two different programs: runAssembly (reads in sff format)...

[NEWBIE] Interpretation of blastp results