Hello,
I'm looking to speak to NGS users about sample preparation pain points, and automation needs. Leveraging novel fluidic solutions,...
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Novel Automation - Feedback Welcome
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help? cDNA variability
Hi all,
I am making my first 10x single nuclei libraries and ran two chromium chips with 4 samples in each chip.
While... -
Illumina Library Prep Webinar
Illumina bietet am 4. Mai 2021 ein Webinar zum Thema Library Preparation an. Das Webinar findet statt vom 10:30 bis 11:30 Uhr (CET) und wir auf Deutsch... -
NGS library preparation robot training
I am wondering the availability of NGS library preparation automation training courses. As I know, PerkinElmer has a limited number of engineers to... -
Is my ATACseq library okay?
We are new to ATAC-Seq. We have performed library validation and several of our samples have produced results similar to those in the image attached.... -
Balance sequencing runs ATAC
I am having trouble balancing my sequencing runs for ATAC-seq. I am doing extensive kapa on the samples but often finding one out of a pool of 6 will... -
Hello from Nairobi, Kenya
Hello everyone! I am Eunice currently working as a Research Associate at the BecA-ILRI Hub, a center of excellence in bioscience research in eastern Africa.... -
NGS library prep facility in Cambridge UK is looking for a Research Assistant
http://www.jobs.cam.ac.uk/job/13622/
We are seeking a molecular biologist with proven skills in RNA/DNA manipulation and a working knowledge... -
ATAC-seq small / overdigested fragments?
Hi all,
First, I hope that the ATAC-Family will grow further to share their experiences here.
My experience with ATAC-sequence... -
Low Concentration Library
Hi,
I made libraries using Colon and Lung Cancer Research Panel and quantify them using qPCR. I was wondering why some of the libraries... -
Library PCR cycle optimisation
Hi there,
This is probably a very naive question, but how can I test the number of cycles required to do library enrichment, if I have... -
Command Line tool or library to work with AB1 files
Hi All.
I have zero experience with sequencing or sequencing files, so please excuse if I ask something stupid
I just... -
How indexes are added in TruSeq Custom Amplicon v1.5?
Hi folks. Could someone tell me that how indexes and sequencing primers are added in TruSeq Custom Amplicon v1.5 library preparation? By ligation (to... -
QIAseq targeted DNA Panel Question
Hi alla!
Anyone has been working with QIAseq targeted DNA Panel??
It is our first trial and I'm not very glad with the libraries... -
nextera xt library prep for bulk RNA-Seq of mouse
Hi All,
We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq...
