Hi,
I was wondering if anyone has experience in demultiplexing BCL files from Illumina with two different barcoding systems in one go?...
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demultiplex BCL data with two different barcoding systems
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Experience with whole transcript vs 3' RNA libraries?
Hi,
I’m planning a large RNAseq project and I’m currently weighting the pros and cons of different library preparation protocols, specifically... -
RIP-seq library prep - single or paired-end reads?
Hi all,
I'm going to be helping a colleague with analyzing some RIP-seq data. We just had a question regarding the library prep protocol. Specifically,... -
How fast is your NGS library preparation?
Everyone wants NGS library preparation to be easy, simple, and fast. The reality is that protocol times for NGS library prep kits vary from vendor to... -
48 v 96 multiplexing
I have approximately 200 samples I'm planning to sequence. I originally planed on multiplexing (Illumina Nextera XT) 96 samples to make two libraries,... -
Postdoc: Molecular and bioinformatic tools for museum and other historical samples
Are you excited about the untapped potential of next-generation sequencing? Do you enjoy making new tools for molecular biology? If so, this postdoc if... -
DNA from separate FFPE slides - to combine, or not to combine?
Hello all,
I've searched the threads but have not found an answer so hopefully I'm not repeating an earlier question.
In the past,... -
Advice needed for shaker usage for illumina library prep kit
Hello Everyone!
In our lab, we will have to prepare library by using Illumina TruSight Rapid Capture Kit. So, according to its protocol,... -
ATAC-seq library stability
I'm just wondering how stable they are after PCR amplification. Recommended storage conditions? How long can the samples survive at room temperature? -
After 1st-strand cDNA synthesis for RNAseq, is it safe to wait a day for 2nd?
Greetings All,
It's my first time doing library prep for RNAseq on a eukaryotic organism, and unfortunately I failed to order 2nd strand... -
Inconsistent size distribution of fragmented mRNA and cDNA for RNA 3Seq
Hello everyone,
I am new to the RNA 3' Sequencing experiment using a protocol developed in house based on Illumina TruSeq mRNA sample prep.... -
Hello
Hi, I am in a R&D group in San Diego, CA and we are working on improving small RNA library preparation workflows using modified adapters to suppress... -
SriptSeq library preparation
Hi,
I am very new to RNA-seq. I have tried making libraries from 5 samples: 3 from eukaryotic RNA and 2 from bacterial RNA. The starting... -
Differential Expression across libraries/platforms
I've been searching for a while now and I can't seem to find any kind of discussion on this topic.
Is there any consensus on the most appropriate...
