Hi all
I recently made ATAC-seq libraries on two new cancer cell lines and I don't see nucleosomal pattern as I used to see in my previous library....
13 results in 0.0029 seconds.
-
ATAC-seq library fragment size troubleshooting
-
Preferred analyzer for library size/library quality
Hello! I am new to the world of NGS and am curious about the preferred instrument for checking library quality. The ones that seem most popular are Agilent... -
MiSeq tolerated library size distribution
Our lab is trying some new library generation protocols where we are size selecting a much broader range of libraries than we have run previously, a 200... -
library depths for sequencing
Hi everybody,
I was approached by a biologist in our lab and was ask a specific question. As I was not really sure, I would like to share... -
Low concentration and large size of Trusight One library
Hi!
I encountered two problems while preparing Illumina's Trusight One library using their guide. I'm following the protocol carefully... -
Low concentration in TruSight One library preparation
Hi!
I encountered two problems while preparing Illumina's Trusight One library using their guide. I'm following the protocol carefully... -
library size calculation using DESeq1/2
Hello,
I would like to know if the lines at the end of the HTSeq file (which includes _no_feature, _ambiguous, _too_low_aQual, _not_aligned, and... -
experimental design for exome sequencing with the new MiSeq
Hello everyone,
my institute recently purchased an Illumina MiSeq (Sep 2012). We want to do exome sequencing with the TruSeq exome capturing... -
Library prep woes – unusual bioanalyzer profile of Illumina RNA-seq library
Hi everyone,
I have been preparing RNA-seq libraries using the Illumina Tru-seq V2 kit. My first Batch of libraries turned out just like... -
Normalization of small RNA data for library size
Hi,
I have a few libraries of small RNA reads with different read counts: some libraries have more than 10 million reads, some others have <1... -
DESeq: estimateSizeFactors, library size estimation
I am working with high-throuput sequencing data and use DEseq to search for diff. exp. genes.
The protocol is somewhat similar to RNA-Seq, but... -
Broad size range for Illumina RNA-seq library - secondary subsampling biases?
Hello!
we are currently having mRNA libraries of non-model organisms prepped and have been running into some issues resulting in very broad... -
edgeR separate offset values/normalizations for each tag
Hi,
Thanks in advance for helping. I'm trying to use edgeR on some RNA-Seq data I have to test for differential expression. However, I...
