Has somebody here figured out how the new "Illumina DNA PCR-Free Prep (Tagmentation)" library preps work?
https://www.illumina.com/p...
11 results in 0.0022 seconds.
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How does the "Illumina DNA PCR-Free Prep (Tagmentation)" work?
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Expired extension-ligation mix TruSight
Did anyone have a problem with expired (expiration 02/2019) ELM4 extension-ligation mix during the library prep for TruSight Myeloid panel? I have a kit... -
Mechanism of ion torrent Adapter ligation, Use with KAPA Hyper Plus Library Kit?
Let me preface this by saying I have never worked with an Ion torrent yet, but we just got an Ion S5, so I want to put it to good use.
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Ligation of Illumina adapters to custom adapters
I would like to create libraries with the following protocol but the second adapter ligations seems not to work:
1. MseI digestion of genomic... -
qPCR vs Qubit, big difference
We have prepared a TruSeq DNA PCR-free library, and quantification with Qubit gives roughly 10 times higher concentrations than qPCR (range 4-50 times... -
How to fill the gap after tagmentation? Can I use Klenow fragment?
I am trying to making Illumina sequencing library using transposon based method to add adaptor. However, instead of using the adaptor used in Nexera kits,... -
Absence or incomplete ligation for SOLID lib prep.
Hello all,
I'm making fragment librairies on a Solid 5500XL, using LifeTech protocols and standard kits) and i am experiencing troubles... -
New NEXTflex DNAseq Library Prep Kits and Barcodes
Bioo Scientific launched the NEXTflex™ DNA Sequencing Kits and NEXTflex™ DNA Barcodes, designed to provide flexibility and high-throughput...Last edited by Bioo Scientific; 06-03-2014, 11:23 AM. -
adapter ligation - Illumina
How can I know if the adapter ligated?
For three of my samples (we are 6-plexing) I forgot to anneal the adapters, however all the samples... -
Ratio in Adapter-Ligation
Dear all,
we design our Adapters and Primers for the Illumina Library by ourselves. Its working very good. The only problem we have is... -
What is at the ends of sonicated DNA?
Given the poor molar yields of typical next gen library construction methods, I have often wondered whether end-repair after shearing was an issue. There...
