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Introductory NGS data analysis & variant calling workshops (online and in-person, early 2026)
For those looking for a structured introduction to NGS data analysis and DNA variant calling, we're running the same introductory workshop in two for...
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Next-Generation Sequencing Data Analysis: A Practical Introduction (September 2-4 in Munich, Germany)
Quality Control, Read Mapping, Visualization and DNA Variant Analysis
Advance your research. Understand NGS and analyze sequenced... -
Registration OPEN: 8th Berlin Summer School in NGS Data Analysis (June 10-14, 2024)
8th Berlin Summer School in NGS Data Analysis
When? June 10-14. 2024
Where? Berlin, Germany
Link? www.e... -
FINAL CALL: RNA-Seq Data Analysis Workshop (March 11 - 14, 2024 in Berlin, Germany)
Exciting News from ecSeq Bioinformatics GmbH!
We're thrilled to announce the return of our RNA-Seq Data Analysis Workshop in March!
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NGS Data Analysis Workshops 2024
Exciting News: ecSeq Bioinformatics 2024 Workshops Are Here!
We're thrilled to announce our diverse range of bioinformatics workshops for... -
Help with htseq -count read counts
Hello
I am doing a transcriptome analysis on Pseudomonas putida and I have been trying to do a read count using Htseq -count. The program... -
mapping quality scores in samtools view?
Hello!
I am using samtools view to map short reads (-phred33) against a reference genome.
The problem is that I don't know how to properly...Last edited by ampsevilla; 05-18-2023, 03:23 AM. -
QC, Alignment, and Mapping: Recommended Tools for Next-Level Sequencing Analysis
With new tools and computational resources being released regularly, it can be hard to determine which are best suited for the analysis process and which older tools continue to be maintained. In an effort to assist the sequencing community, we interviewed three highly skilled bioinformaticians about their recommended tools for several important analysis applications.
Quality control and preprocessing tools
“Garbage in, garbage out” is a popular... -
Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation
Can anyone suggest if there is any comprehensive snakemake or Nextflow workflow for ONT reads QC, Assembly, Polishing and Evaluation? -
Insertions follow reference in vcf file
Hi,
I'm working with gvcf files, containing also non-variant positions. I have noticed some insertions that I'm not able to correctly interpret... -
Online Course - A Practical Introduction to NGS Data Analysis (September 26-28, 2022)
Online Course - A Practical Introduction to NGS Data Analysis
Quality Control, Read Mapping, Visualization and DNA Variant Analysis
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directional vs nondirectional methylation libraries
Dear all,
I am currently working with QiaSeq Methyl data. I was very surprised that my bismark/bowtie pipeline, which worked fine so far... -
Can I use only one read from paired end reads for breseq analysis?
Hello
This might be a stupid question, but I have just discovered tool called Breseq for microbial variant and SV analysis. The problem... -
Stampy software error with PairSorter
Hi all,
I am trying to map reads (Paired End) from a sample to a reference assembly that is somewhat divergenct (~3%). So I used the bam file... -
SUBJECT: Stampy software error with PairSorter
Hi all,
I am trying to map reads (Paired End) from a sample to a reference assembly that is somewhat divergenct (~3%). So I used the bam file...
