15 results in 0.0022 seconds.
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Nextera Mate Pair library: expected size after tagmentation
Hi! I'm having problems with the tagment genomic DNA step of the Nextera mate-pair library preparation kit and I really don't know if the enzyme is working... -
Uploading MiSeq mate-pair in CLC Genomics Workbench
Dear all,
I'm trying to import MiSeq FastQ files to CLC Genomics Workbench (version 4 point something), and I want to make sure that I'm... -
how many chimera could be generated by circulation process of mate-pair preparation?
Im currently thinking to apply mate-pair library in my metagenomic project on cellulose-degrading microorganisms using Illumina Hiseq 2000 platform. but... -
Are there some human mate-pair data available online for public use
Are there some human mate-pair data available online for public use.
I want to test my mate-pair data analysis pipelines. It's better if it is... -
For Nextera mate-pair kit, Is it possible to reduce the tagmentation reaction time?
For Nextera mate-pair kit, Is it possible to reduce the tagmentation reaction time to get more long fragment, say 10kb? we have use this kit and found... -
Newbler with fastq from sff
I know this is an odd request, but here goes:
I want to be able to extract an 8KB sff to paired fastq files, and then assemble those fastqs... -
scaffolding GAII paired-end library with Hiseq mate-pairs
Hello,
we are a Belgian research team studying the +/- 4Mbp genome of a bacterial plant pathogen (and newbies in NGS data analysis). We are getting... -
Samtools Flag to identify outward facing (mate-pair not paired end) reads
Hi,
Can someone tell me what flag I should grep for in order to
obtain reads that are outward facing (mate pairs: <--- && --->... -
Library preparation
Hello everybody,
I would like to know what is the difference between the mate-pair and paired-end preparation for NGS and how these results... -
Mate-Pair Shearing
Illumina suggests hydroshear or nebulizer for the first shearing in the mate-pair library protocol. I was wondering if anyone has used a covaris for... -
Mate-Pair Illumina library problems
Hi, I am about to start a resequencing project on the HiSeq 1000 searching for rearrangements in a bacterial genome.
This is the 1st time I am... -
BWA behaviour with Mate Pair data + Multi read mapping
Hi All
PS: This message was also posted on BWA mailing list but I did not get any response.
I am not able to logically... -
anyone with expertise on cDNA mate-pair library?
Would like to know, if anyone has made a mate-pair library (preferably for Illumina) using RNA as the starting material. I found a PET protocol (454)... -
Aligners for Illumina's mate-pairs
Hello everybody,
I just started working with mate-pair data from Illumina and I am a bit lost regarding which aligners can actually work... -
Closing gaps using Solexa mate-pair data
Does anyone know any software that will close gaps between contigs using Solexa mate-pair data (insertion ~2500kb)?
