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CAP3 - strange behaviour, never finishes running (2xXeon 2620v4/Ubuntu 16.04.2 LTS)
I am running CAP3 under Ubuntu 16.04.2 LTS (2xXeon 2620v4) to assemble a very small data set (31 contigs in 7k-700k bp range). I am doing that to check...
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Number of Reads Increasing
Hi there,
I trimmed my reads and then merged them using FLASh. However, I found that the number of reads before processing (trim and merge) is...
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Adaptor Removal with Trimmomatic
Hi there,
I ran a HiSeq2500 run and found that I had adaptor sequences still present.
I first used Trimmomatic to simulatenously...
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Illumina HiSeq2500 Pipeline Question
Hi there,
I have just received my Illumina HiSeq data back. I have 2x250bp runs (so forward and reverse reads).
I ran a FASTQC and...
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Can I use tabix on vcf.gz files?
As a beginner I might have a stupid question, but I have several VCF files of different individuals that are zipped ( .vcf.gz). I want to merge these...
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Problem in merging 1000G and cases in Plink
I have some problems in stating the imputation. I work on imputation in case-only design. My reference panel is build37 of 1000G (phase 3) in VCF format...
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FASTA + FASTQ --> "FAST(q)" + FASTQ
I need some help out here with an actually not that difficult task and useful to my field of research. Unfortunatley I cannot find a tool which makes...
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Concatenate / merge IPS XML files
Hi,
Does anyone know how to merge, or concatenate InterProScan XML files into one large file?
Thanks,
Andor
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merge .biom files
Hi,
I would like to ask you if it is possible to merge .biom file.
Maybe it is not possible directly but starting from the bam files?...
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Velvet merged dataset
Hi, my issue is that I recieved data which had already been merged and trimmed and so I have binned it to smaller sizes and am about to finally assemble...
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SAMtool merge/cat problem
Hi guys,
I d like to cat or merge 2 BAMs file. My problem is that I try to cat the two BAMs but when I ve used IGV to see the result the...
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how to merge de novo transcriptome assemblies
Hi all,
I am working on building de novo transcriptome assemblies using Trinity. In the future, I would like to merge assemblies, so that...
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Merging paired end reads (R1 and R2 files)
To anyone who may have dealt with Illumina MiSeq paired end reads: what are the best programs/scripts to use when merging the R1 and R2 (forward and reverse)...
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minimus2 assembly merge
Hi folks,
I'm using minimus2 to merge two different assemblies - a long read (LRseq - moleculo data) assembly done w/ celera and an idba_ud...