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I am trying to find the most significant altered genes and I was thinking of using only 1 tool for significance test which is MutSigCV instead of MutSigCV for mutation data and GISTIC for CNV data. I know that MutSigCV works with short indels but can I merge my CNV segmented data in the MAF file (Divide each long CNV into regions) and input them to MutSigCV or this wouldn't work? Can MutSigCV work with long insertions or deletions? What is the maximum indel length that MutSigCV can operate on?
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Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM