Hello all,
I keep facing crash while mapping contig files with BBMap(v.38.76). I am running command "bbmap.sh in=sample_r1.fastq in2=sample_r2.fastq...
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bbmap.sh: line 352 RANDOM# Killed
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summary statistics of metagenomics
Hey All
I want to represent my 200 metagenome assemblies and preliminary analysis in a table. I was wondering if the following data are...
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Processing a very large soil dataset
I’ve been tasked with assembling a very large soil metagenomic dataset, so large that I’m having second thoughts about my regular processing pipeline,...
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Metagenomic Assembly from PacBio reads
Hi everyone,
I am trying to assemble a bacterial genome using PacBio reads and short illumina reads from a metagenomic pool of both eurkaryotic...Last edited by lmbrutscher; 04-03-2017, 12:21 PM.
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Clustering/Binning Metagenomic contigs with MyCC and 16S sequences
I have tried to use MyCC (http://www.nature.com/articles/srep24175) on samples with varied diversity and I always get very good accuracy between number...
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Assembler for denovo metagenomes
I have two environmental samples that have been sequenced on Illumina MiSeq (2x250). First sample has about 200 Mbp (4M reads) and second has 400 Mbp...
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Shotgun Metagenomics data analysis
Hi,
I am new to shotgun metagenomics analysis. I have a project on the human gut microbiota. I know about MG-rast but I want to analyze...
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Assembled contigs vs short reads
I have recently finished assembling some metagenome sequences and after assigning function to my contigs I see that most genes belong to three specific...
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Introducing BBNorm, a read normalization and error-correction tool
I'd like to introduce BBNorm, a member of the BBTools package.
BBNorm is a kmer-based normalization tool for NGS reads, which may also be...Last edited by Brian Bushnell; 01-29-2015, 09:47 AM.
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metagenome scaffolds
Dear all,
I have 3 illumina metageome sequence files of the same sample, with different insert size in each. Insert sizes are 300, 400...