Good Morning All,
I am trying to sequence a few hard-to-ID Enterobacteria using the Oxford Nanopore Rapid Barcoding Kit (SQK-RBK004) on the...
28 results in 0.0022 seconds.
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Pooling for Oxford Nanopore Multiplex Sequencing
Last edited by hathormaat; 03-16-2022, 07:49 AM. -
Discrepancy between Qbit quant & read count - possible contamination?
We are performing MinION sequencing of DNA extracted from fresh water samples.
We got some preliminary results that suggested possible contamination,... -
Using the rapid sequencing kit for cDNA
We haven't done RNA-seq with the minion yet, but have an extra Rapid Sequencing kit lying around. I am wondering if there's anything "wrong"... -
MinIon Prep Kit Selection (Ligation vs. Rapid)
Hey all,
I'm looking to sequence a handful of pure bacterial isolates for WGS and de novo assembly. Our group has decided to go with a combined...Last edited by hathormaat; 10-06-2021, 10:39 AM. -
errors at the beginning of hybrid assembly using MaSuRCA-4.0.3
Hi
im trying to make hybrid assembly for Ecoli using MiSeq and Nanopore data in Masurca. currently i m getting two errors marked in Bold... -
Space/microgravity simulation: Extract DNA for MinION
Hello,
I hope to collect samples from lava tubes (microbial mats on the walls and nearby soils) to perform a metagenomics analysis. I will... -
Nvidia drivers login loop plus flickering/blinking screen-Ubuntu 18.04 Linux
Got stuck in the login loop. I hard restarted the computer....went into the terminal and was having some path issues (I wanted to uninstall nvidia drivers).... -
docker installation for Guppy basecaller
Linux
Ubuntu 18.04
nvidia-docker2 (2.0.3+docker18.09.7-3).
Nvidia driver 410.57
cuda 10.1
error occurs aft... -
ssDNA sequencing on MinION without PCR or doublestranding
Hey people!
A colleague of mine wants to sequence a library of ssDNA Adeno-associated viruses on the MinION to get a distribution of their... -
Demultiplex nanopore reads with custom barcodes
We have a problem trying to demultiplex MinION sequences with custom barcodes. Do you have any software recommendations we can try for demultiplexing... -
Using Basic MinION Starter Pack in remote area for soil samples
I am not experienced with metagenomics/metatranscriptomics/soil microbiology/Nanopore technology, so please forgive if this post is misguided
... -
Minion sequencing
Hi all,
I have a very basic question. We have ±10 samples that we would like to sequence using MinION. These are PCR amplicons that we... -
Flongle flow cells - now shipping
Oxford Nanopore Technologies have started shipping Flongle adapters and flow cells to customers. These are disposable flow cells using fewer pores and... -
best approach in dealing with low input DNA
Hi everybody,
we already posted this in the Nanopore forums, but we wanted to have a better chance of receiving answers so we post it again here.... -
Question on multiplexing - long reads
Hi All
So I have 2-3 number of 2MB bacterial genome and two plasmids of 17 kb each length. I am looking at the feasilbility of multiplexing...
