Have a strange situation, our latest MiSeq run failed for 16S - from a protocol (EMP @ ArgonneNatLabs) that we use all the time.
The clustering...
18 results in 0.0022 seconds.
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MiSeq run failed; low clustering, but good QC
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question re: sequencing past lesion
I used miseq for a funky purpose: I wanted to know what a particular polymerase is incorporating opposite a defined lesion in a synthetic construct....Last edited by anzo; 06-03-2019, 11:44 AM. -
V4-V5 Region Using 2x250 - Enough Overlap?
Hi All,
We've been looking at the V4 region (515/806) in environmental samples for some time successfully. We'd like to move to the V4-V5... -
Quality per tile
Hi there,
I have three samples which were sequenced on a MiSeq. I have attached the R1 and R2 quality per tile for sample 3 and R2 for... -
Sequencing depth
I am sequencing samples from animal gut using illumina MiSeq/HiSeq, targeting the v4 region of the 16S
I get 22m raw reads back from MiSeq... -
MiSeq maximum fragment size
Hi there, does anyone know if there is a maximum size fragment that won't be sequenced by a MiSeq? The approach is paired end 250x250 on a size selected... -
MiSeq 2x250bp run
Hi there! I have a silly question about the MiSeq read length.
I'm going to run a MiSeq plate of 16s amplicons.
When they talk about 2x250bp... -
Can we stop the run and re-generated the cluster on flow cell on MiSeq?
We are using the MiSeq machine (2*250, V2 kit) for a Truseq library sequencing. As we find the cluster density is low (only 239k) on the cycle 3, can... -
16S V4 amplicon concentration after first PCR step
Dear all
I am analyzing gut samples from fish using illumina's 16S protocol, amplifying the v4 hypervariable region. I am getting ok bands... -
Illumina Long ReadKit (Moleculo) on MiSeq
Has anyone taken the moleculo kit and run on the 250bp runs on the Miseq and tried the assembly after? I'd like to know if it would work. On the Illumina... -
Problem extending/scaffolding contigs with MiSeq 250bp reads?
Greetings. I have some denovo assembled contigs (Velvet 1.2.10) made from 250bp MiSeq (Illumina) reads. After I make the contigs, I normally extend... -
Assemble
Dear Friends,
Hey friends I want to know that how to perform assembly of Miseq data? Is there any tool or it is possible... -
High Percentage of Soft Clipping, BWA-MEM, MiSeq
Hi everyone,
I have 1,377,239 paired reads, 250bp each, 82,486 of them have been soft clipped for more than 200bp (eg a read with CIGAR... -
SOAP denov "missing reads"
Hi there,
my question is about denovo assembly using SOAP denovo-trans. I have data from a MiSeq, 20 Mio paired end reads, 250bp each,... -
overlapping in PE library sequencing
Hello,
I'd like to do de novo assembling of a fungal genome(~20 Mbp).
I will use Miseq data for the assembling.
I found...
