Hi all,
I am having problems with the NEBnext Ultra kit for library preparation. I´ve been doing same amplicon libraries for three years with...
14 results in 0.0024 seconds.
-
NEBNext adaptors clustering failed
-
Hey, let's talk about mate pairs and MiSeq
Hi all, I'm new to posting but have been using this site for reference for about a year now.
I'm analyzing a bacterial genome sequenced on...Last edited by MicroMicro; 12-20-2016, 02:08 PM. -
Terrible overclustering -- 16S EMP protocol
Hi SEQ-users,
We've been using the following protocol for 16S V4 region amplicon sequencing on MiSeq, which I think is very familiar to... -
demultiplex FastQ files based on read names
Hi everybody,
my name is Fabian Roger and I am a PhD student in Ecology at the university of Gothenburg.
I am at a stage... -
Sequencing depth
I am sequencing samples from animal gut using illumina MiSeq/HiSeq, targeting the v4 region of the 16S
I get 22m raw reads back from MiSeq... -
MiSeq 2x250bp run
Hi there! I have a silly question about the MiSeq read length.
I'm going to run a MiSeq plate of 16s amplicons.
When they talk about 2x250bp... -
MiSeq amplicon: corrected int spike
My last MiSeq 16S amplicon run (2x250) and a rerun with a lower cluster density both showed large spikes in corrected intensity (>1000) during the...Last edited by sharchaea; 05-26-2015, 08:51 AM. -
16S library issues
Hello to all of you.
I am a newbie in the genomics field, so I address here my problem hoping for some feedback and solving ideas.
I am... -
amplicon seq, 2 step, nextera indices vs homebrew
Hi all
Currently we are sequencing 16s amplicons on our MiSeq using the Caprasso primers. I have people asking to do a variety of non-16s... -
16S V4 amplicon concentration after first PCR step
Dear all
I am analyzing gut samples from fish using illumina's 16S protocol, amplifying the v4 hypervariable region. I am getting ok bands... -
Why is a seventh base needed with MiSeq 6-base indices?
The MiSeq sample prep guide indicates that a seventh base is needed after the 6th base. What is the reason for that?
... -
'Hallo' from Germany!
Hi all!
I'm a PhD-student in Germany and work with small eukaryotes that thrive within ocean currents (Plankton). In one of my projects... -
Ampliseq panel on MiSeq
Hi I have made an Ampliseq panel for use on the PGM, it has 280 targets of sizes between 250-150bp.
We would like to also sequence these same... -
Variable amplicon size for MiSeq, bias? Maximum size?
Hi all!
We want to sequence amplicons on the MiSeq to get (~200 bp) sequence of an exon of a specific gene. However due to variability...
