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superpyrin started a topic MiSeq errors on scFv linker

in Sample Prep / Library Generation

06-22-2016, 04:42 AM
MiSeq errors on scFv linker

Hi, we used MiSeq 250 to sequence amplicon library of scFv antibody variants. Heavy and light chains are connected by a peptide linker (HeavyChain-GGGGSGGGGSGGGGS-LightChain)....

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