Hi,
I want to use digital normalization on a set of single cell sequencing data as well as metagenomic date from low complexity communities....
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kmer size and coverage cutoff for digital normalization using the khmer suite
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Combine TruSeq DNA PCR-Free library and 2x300bp PE sequencing on MiSeq
Hi everyone,
We are about to do whole genome sequencing of an 12Mb organism. There are still a lot of "loose ends" in the reference... -
16s MiSeq Illumina library concentration and PhiX
Hi all,
I have 2 small questions concerning the 16s sequencing protocol for MiSEq:
1) Some samples have very low concentration... -
Nextera XT fragment size and MiSeq v3 read length clashing ?
Hi all,
I am planning a bacterial genome sequencing project (5 Mb) for around 80 samples to get good coverage. As the input material is...