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Convert mothur classify.seqs output to BIOM format
I got a lot of problems trying to convert output of mothur classify.seqs to BIOM format so that I can import data into phyloseq R package. I have FASTA...Last edited by Petr Kozyrev; 04-30-2017, 08:09 AM. -
MeFit - does it work?
Hello, I am new to NGS analysis and am considering using MeFit to merge and filter my 16s rRNA data from MiSeq. I requested PE300 (now I regret it) for... -
Trouble: getting reads with Reverse primer
Hi SeqA team,
I've hit another road block.
Data information: 16s V3-V4 region, demultiplexed. Illumina, Paired end. PhiX 5%... -
Very large distance matrix using MOTHUR
Hello,
We are trying to process a large dataset (~200 samples, each with ~100,000 Illumina 250bp 16S reads) using MOTHUR. We now have... -
guidelines with 16s RNA data
Hello Members, and Seniors,
I don't have any experience in/on 16s RNA data.
I know there are tools like QIIME, and mothur which do...Last edited by bio_informatics; 05-01-2015, 04:55 AM. -
MiSeq V4 16S run low OTU evenness
Hi everyone
Recently received my sequences back from an Illumina MiSeq 16S V4 300bp PE run, performed by a biotech company. The DNA was... -
MiSeq - 16S - what to do with unassembled reads?
Hi,
I'm working with 16S data, and have been using PEAR (http://bioinformatics.oxfordjournals...formatics.btt5... -
cluster and cutoffs using Mothur
Hello,
I've been using QIIME and Mothur to analyse different sets of 454 data, and this is a little off topic, because it's related to... -
Mothur and fastq file format
Hi all.
I am completly new at NGS. I have just got back my datas from a first run of 454 pyrosequencing. We want to get into metagenomics... -
problems with MiSeq data using GAIIx primers
Hello,
Our lab has just recently sequenced 16S DNA using the MiSeq platform. We had previously sequenced using the GAIIx platform and were...Last edited by user19; 02-04-2014, 05:21 AM. -
using Mothur to demultiplex plate prepared using RLMIDs
Hi,
I am trying to switch over from the Roche tools (sfffile, sffinfo..) to using Mothur to demultiplex some metagenomics data. However one plate...
