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mac656 started a topic Cheapest cell lysate --> mRNA strategy

in Sample Prep / Library Generation

06-02-2015, 10:25 AM
Cheapest cell lysate --> mRNA strategy

Hi,

Can anyone fill me in on the most cost-effective strategy from going from cell lysates to mRNA, either directly or indirectly? Currently,...

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chin0304 started a topic CAPPED-mRNA sequencing

in RNA Sequencing

01-29-2015, 09:44 AM
CAPPED-mRNA sequencing

Hi,

Has anyone tried to enrich for capped-mRNA (of virus)vfrom total mRNA?
if yes, what kind of method did you use?
I know...

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SeqByChoice started a topic Drosophila mRNA purification, 18s trouble!

in Sample Prep / Library Generation

02-10-2014, 02:59 AM
Drosophila mRNA purification, 18s trouble!

Hi,

using Dynabeads mRNA purification kit (polyA, LifeTech), on Drosophila total RNA, we are always left with about 11% rRNA. Primarily...

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