Hello everyone,
Kindly help me how to find a virulent strain of a virus by aligning the sequences in the databases.
Thank you...
23 results in 0.0030 seconds.
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Get same start position like reference
Hi, i am wondering is there any software to rearrange finished genome has same start position as reference? Because I want to do Multiple Sequence Al... -
Rearrange Scaffolds after De Novo for Multiple Sequence Alignment
Hi, Right now I have 3 chloroplast genomes. I want to do rearrange the scaffold based on reference so I can do Multiple Sequence Alignment. Is there any... -
MSA on large scale of sequences?
Hello guys,
I have 15 miRNA seq, around 8MB each. What i am trying to do is, apply Multiple Sequence Alignment and get a consensus sequence... -
How can I filter out reference sequences that get multiple hits in BWA MEM ?
Hi everyone !
I have exported a small number (~500) of 145 pb sequences from a Stacks analyse.
I then used it as my "reference... -
a lot of multiple alignment reads after rRNA depletion
I met the situation of high % reads with multiple alignment sites. Our procedure is removing rRNA by Ribozero kit from ~1 microgram RNA isolated from... -
Total RNAseq multiple mapping issue
We are running paired-end total RNA (using blood) on HiSeq and after adapter and quality trimming (Q>30), we use tophat2 for alignment. We are getting... -
High multiple mapping rate Drosophila pair-end 50 bp RNAseq
Hi, I used Tophat2 for alignment. The mapping rate is ~92% while the multiple mapping rate is ~25%. Based on the FastQC result, it has some problem in... -
Need help to edit multiple sequence alignment?
I have a large concatenated protein multiple sequence alignment. One of the species have many undetermined 'X' amino acid. Such column will probably not... -
HUGE differences between multiple sequence aligners.
So I have 2000 sequences I am aligning to identify comonalities. Firstly I am using different MSA programs (clustalo, muscle and MAFFT) and then converting... -
MSA with 80,000 30bp seqences, provides 1500 base in output file
I have been trying to do a multiple sequence alignment with 80,000 sequences to identify any similarities. All bases are 36-37bp long however when I run... -
How to implement Clustalw in Perl Program
Please be kind to suggest how to implement clustalw in a perl program.
What all steps I need to follow.
Also , I have referred to... -
Using lastz (FAILURE: in load_fasta_sequence)
This is my lastz command
$ lastz hg19/chrAll.fa[multiple] merged.fsa
Error
FAILURE: in load_fasta_sequence for hg19/chrAll.fa,... -
help with bio perl
Hi,
I am handling different formats of Multiple sequence alignment files.
I can get the format of file by
...Code:my $format_guess
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Tool for counting mismatches in hundreds of files?
Hello, I need to align 2 sequences in hundreds of files and then write a list with the count of the mismatches in each file.
I thought...
