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The order of multiple hits of a read in Bowtie
Hi, I am using bowtie for the alignment and find that for the reads with multiple hits, the first alignment is more likely (but not always) to be the...
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Bowtie XA, X0, X1 tags in SAM output
Hi Everyone,
Background: I've been using bowtie and samtools for a couple years. In the past 6 months have I converted to outputing SAM...
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multiple alignments with BWA
The manuscript "Fast and accurate short read alignment with Burrows–Wheeler transform" says this:
"It [BWA] performs...
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How many multiple alignments to allow?
Hi everyone, I am working with human transcriptome sequenced by SOLiD - at the moment single read, but plan to try mate paired next - and using Bowtie/TopHat/Cufflinks...
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question about bwa sam file
Hi, Everyone
I used bwa samse to generate a sam file (all of setting are default, including index and aln steps), but I got some lines like...
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BWA sampe multiple hits question
Using sampe n=10 and N=0, I get the following results.
seq#0 83 gi|150002608|ref|NC_009614.1| 834297 37 75M = 834220...