Hi, Everyone
I used bwa samse to generate a sam file (all of setting are default, including index and aln steps), but I got some lines like this:
301 VHE-8239602811014-9-2-3-1761 16 uc008guk.1 3521 23 16M * 0 0 AGTGCTCAGAGATGTA * XT:A:U NM:i:1 X0:i:1 X1:i:1 XM:i:1 XO:i:0 XG:i:0 MD:Z:13C2 XA:Z:uc009jck.1,-72,16M,2;
Since X0 means 'Number of best hits' based on bwa manual page, in my case, it's X0:i:1, this should be only one, but it does has XA field, XA:Z:uc009jck.1,-72,16M,2;, which means it has another best match. Any comment? Also, in XA field, -72 should be position, but why is that negative?
Thanks a lot
Gilbert
I used bwa samse to generate a sam file (all of setting are default, including index and aln steps), but I got some lines like this:
301 VHE-8239602811014-9-2-3-1761 16 uc008guk.1 3521 23 16M * 0 0 AGTGCTCAGAGATGTA * XT:A:U NM:i:1 X0:i:1 X1:i:1 XM:i:1 XO:i:0 XG:i:0 MD:Z:13C2 XA:Z:uc009jck.1,-72,16M,2;
Since X0 means 'Number of best hits' based on bwa manual page, in my case, it's X0:i:1, this should be only one, but it does has XA field, XA:Z:uc009jck.1,-72,16M,2;, which means it has another best match. Any comment? Also, in XA field, -72 should be position, but why is that negative?
Thanks a lot
Gilbert
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