Hi,
We are about to start a fairly large experiment with the Nextera Flex kit in a very limited time. After some testing and reading experiences...
56 results in 0.0044 seconds.
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1/5 Nextera Flex number of cycles
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Adapters for Nextera Mate Pair Library Prep Kit
Dear,
I have a question about Nextera Mate Pair Library Prep Kit (https://www.illumina.com/products/by...its/library-pr... -
Ordering Nextera XT Oligos from IDT?
I would like to start ordering Nextera XT Index Kit v2 Set A from IDT in plate-based format, instead of plating them out myself. I called them hoping... -
Recipe for NexteraXT respuspension buffer?
So, I will be running out of resuspension buffer (RSB) for my NexteraXT kit before everything else...
Does anybody know what is in it or... -
Post-demultiplex adaptor removal?
If one has demultiplexed dual index reads on a MiSeq AND included "adaptor removal" as part of the demultiplexing (on instrument), should one... -
Pooling and sequencing NexteraXT and TrueSeq PCR free libraries on a single lane
Hi everyone,
I have a question regarding the pooling of NexteraXT and TrueSeq PCR free libraries onto a single Illumina HighSeq lane. I... -
Combine Nextera and Nextera XT in one lane
Hello!
We are preparing libraries for another HiSeq 4000 run and would like to combine samples prepared with both Nextera and Nextera... -
align reads generated with Nextera exome kit (designed on GRCh37) to GRCh38?
Dear all,
in my lab we are using the Nextera Rapid Capture Exome kit to do whole exome sequencing. Until now I have used GRCh37 as a reference... -
script for checking barcode compatibility
Hi everyone,
a colleague of mine wrote a script that checks the compatibility of barcodes used in different sample prep kits for Illumina... -
Nextera Mate Pair library: expected size after tagmentation
Hi! I'm having problems with the tagment genomic DNA step of the Nextera mate-pair library preparation kit and I really don't know if the enzyme is working... -
nextera xt library prep for bulk RNA-Seq of mouse
Hi All,
We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq... -
Tapestation quality peaks show unwanted larger fragments.
Hi all,
I used Covaris to shear human genomic DNA at a set size of 300bp and 200bp respectively. This is to sequence my samples...Last edited by mzahir89; 08-19-2015, 02:20 AM. -
Nextera XT for prepping Chip-Seq sample?
Hello,
Illumina suggests using their TruSeq ChipSeq kit, but I wonder if the Nextera XT should work as well for chipseq sample (mainly because... -
Squeezing the most out of Nextera XT...?
Hi All,
I'm wondering if anyone has experimented with pushing the limits of Nextera XT in order to get more samples out of a kit? I want... -
Sequencing Long-Range Amplicons affordably?
Hi All,
I have a question regarding sequencing long-range amplicons affordably, as the title suggests! So, in short I have about 40 long...
