Hello,
I'm preparing libraries with Nextera XT. From 40 samples 7 are not alright. They are shorter than the rest of my libraries and I'm unsure...
44 results in 0.0066 seconds.
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Apply "Nextera" enzyme for RNA-seq library
Hi all,
Recently I'm trying to make RNA-seq library using Nextera XT kit.
However, I noticed that kit shipping takes 1 month.
As I... -
Trinity Adapter Trimming: Clontech and Illumina?
Apologies for what is an often repeated topic, but with (I believe) a new twist.
I have several RNA libraries that I amplified with the... -
Quantitation of Nextera XT library
I have searched the forum without success so apologies in advance if I have missed this elsewhere.
We are sequencing pooled Nextera XT... -
nextera xt for 30 Mb genome
Hi all,
I was wondering if anyone has experience or has tested using the nextera xt kit for moderately sized eukaryotic genomes (20 - 40... -
Recipe for NexteraXT respuspension buffer?
So, I will be running out of resuspension buffer (RSB) for my NexteraXT kit before everything else...
Does anybody know what is in it or... -
NexteraXT Bionalayzer - undertagmentation?
Hi,
I am using the NexteraXT kit to prep mtDNA. I just did a test prep of some samples and had them run on a HS DNA Chip on the Bioanalyzer... -
non-equimolar pooling to normalise read depth in libraries with different genome size
I'd like to sequence libraries of very different genome size on a single MiSeq run.
Eight bacterial samples with 4.5Mb genome size
... -
Combine Nextera and Nextera XT in one lane
Hello!
We are preparing libraries for another HiSeq 4000 run and would like to combine samples prepared with both Nextera and Nextera... -
Mixing pooled libraries at the same time
Hello everyone,
I am a new member of SEQanswers. I am planning to run MiniSeq system for the first time. Unluckily, I have a big problem.... -
MiSeq Pooling\multiplexing 240 samples using Nextera v2 Index
Hi everyone,
I am following Illumina's 16S protocol for sequencing and I use Nextera XT Index v2 adapters kit. I do my runs on MiSeq using... -
Overcoming PCR Primer Bias with MiSeq?
Hi, I am a grad student new to MiSeq and HTS in general. I'm trying to figure out which sequencing kit/method to use for my project, and I'm getting some... -
nextera xt library prep for bulk RNA-Seq of mouse
Hi All,
We purchased an illumina nextera xt DNA library prep kit and accompanying 96 index kit with the goal of doing single cell RNA-seq... -
What is in Illumina's Normalization Beads?
Illumina's normalization beads have formamide, are very stinky, must be used in a hood and use NaOH to render the DNA single stranded. I looked into alternative... -
Nextera XT tagmentation problem
Hello all,
I need some help with Nextera XT library prep from bacterial genome.
DNA were extracted using QIAmp kit with QIAcube HT robot...
