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Help with ideas for barcode screw up
Got any sequencing wizards out there? Undergrad barcoded 10x 96 well 16S amplicon plates for a miSeq muliplex run with the same set of Nextera barcodes... -
Change standard index sequence in sample sheet for Miseq
We synthesized fusion primers (i.e p7(or p5)-index-Transposase- gene_specific_Primer) using the Nextera adapter and index sequences, and prepared a library... -
Combining TruSeq and Nextera XT Indices
Hello people,
we usually prepare and run the samples on our MiSeq using the 26 6nt-long i7 TruSeq DNA Indices (formerly TruSeq LT, I think).... -
index diversity on MiSeq
Can I run 2-3 index combinations on MiSeq? I'd be using Nextera XT DNA sample preparation index kit and MiSeq v2 reagent kit. If I select index pairs...
