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NexteraXT kit on cDNA amplicon?
Can I use the Nextera XT library prep kit on a 2kb cDNA amplicon? Just wanting to check that the tagmentation process will work ok on cDNA. I've only...
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Cleaning paired-end Nextera prepped reads
These reads were sequenced (2x100) with Hiseq 2500 rapid mode. In the first pictures, the reads as they were when I got them:
R1
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Illumina Miseq primer TM and library preparation for fungal metagenomics
I have previously worked with 454, but fairly new to Miseq platform. Using Nextera XT sample preparation kit, I am going to study soil and root fungal...
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Nextera XT for Normalized full-length dscDNA
Hello everyone,
I am try to make illumine Hiseq 2500 library using normalized full length dscDNA as the start materials. Someone suggested...