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How are you liking your NextSeq 2000?
My team has reached the "serious considerations" stage of purchasing a NextSeq 2000, and I'm curious to hear how others are liking (or disliking?!)...
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Large Library On Multiplexed Run
Hey all,
I'm currently running highly multiplexed runs on a Nextseq 550 and I've come into the problem where a library submitted to me...
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Problems sequencing a low diversity library on NextSeq
Hi everyone,
We’ve done several attempts at sequencing a low diversity library on a NextSeq Mid Output v2.5 flow cell, following the...
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NextSeq Camera functionality
Hi,
Is there a NextSeq log file/metric that captures metrics that would show the cameras are working and in focus during a run? It seems...
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nextseq 500 - service discontinued?
I hear rumors that service on the Nextseq 500 has been discontinued. Has anyone had issues getting service contract, parts or reagents on their Nextseq...
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Reduce PhiX
Dear all,
I was wondering if there was any tool to help me reduce the PhiX% used in our NextSeq 500 runs? We run similar samples continuously,...
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Bubbles in R1 only (NextSeq500)
Hi seqanswers! We have a new problem with our NextSeq: showers of small bubbles that appear only in R1 and cause high error tiles on the lower surface....Last edited by Chris Coldren; 10-29-2018, 11:13 AM.
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microserver direct connect to nextseq 500
Is it possible to tether/direct connect via cat 6 to the nextseq500? The idea would be to run bcl2fastq on the server. The sequencing data would transfer...
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Forgot to denature NextSeq library
I forgot to heat denature my NextSeq500 library. Will it still cluster and give enough data or should I cancel the run?
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16S sequencing from total RNA?
I'm working with a researcher that wants to identify the bacterial communities present in human dental pulp tissue, blood, and saliva from isolated RNA....
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third party illumina equipment
Has anyone else had trouble getting Illumina to service or support instruments not bought directly from Illumina? Any feedback on software license transfer?...
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Recommendation 150nt single-end vs 2x75nt paired-end
We are in the planning phase for an experiment that requires sequencing of a DNA pool of length about 140bp. It is crucial that the entire fragment is...
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NextSeq - Banded Clustering images
In September, I noticed that our NextSeq is producing a very odd banded clustering image. Specifically a there's a very bright band of clusters through...
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Can't sequence ATAC-Seq libraries
We have prepared ATAC libraries for 24 samples following the protocol from Buenrostro et.al, using their custom Nextera-like primers and we have attempted...