18 results in 0.0022 seconds.
-
Low-plex pooling: how bad will this combination be?
Hello! This is my first post to SeqAnswers, but I have perused the forums before, so before I start describing my problem, hello to this fantastic community....Last edited by msanthropologist; 12-17-2024, 09:45 AM. -
Novaseq Kit Selection and Metagenomic Shotgun sequencing advice
I have an incoming project to which they would like us to perform metagenomic library prep and sequencing. They intend to give us gDNA environmental samples... -
Illumina (NovaSeq 6000) cluster registration?
Hi folks,
I'm new here, and I'm in the process of preparing a PCR-generated amplicon sequencing library (composed of many samples) that will... -
Asymmetric sequencing on Illumina Novaseq S4 beyond specification | Cycle Transferring
Hello,
I would like to sequence 250 bases on `Illumina Novaseq S4`, on read 2.
1) Kits of illumina come in the following sizes:... -
what is the sequence of Illumina NovaSeq sequencing primers?
Anybody know what the sequence of Illumina NovaSeq sequencing primers are? -
NGS data analysis - help
Hello everyone!
I am a 1st year PhD Biochemistry student who is doing NGS for the first time (also the first time in our research group).... -
Anyone using NovaSeq 2x250 for 16S sequencing?? 1 billion + reads..
Title says it all.
This seems like the future for 16S sequencing but haven't heard of anyone validating yet. Seems like 2x250 is enough... -
Looking for Participants: Meenta NovaSeq RunShare Beta
Hi Everyone,
I'm from a small Boston based company called Meenta, and we are launching a beta for our NovaSeq Runshare Program for a small... -
NovaSeq placement issue
Hi,
has anyone had issues with placement of NovaSeq in LEED buildings or other buildings with variable temp and humidity profiles?
... -
Exact duplicate reads/readnames/quality/tiles in Novaseq FASTQs
Anyone seen this? Some (not correlated with software versions AFAICT) of our MarkDuplicates jobs are failing because there are exact copies of the same... -
NovaSeq uneven lib sizes: any tagwise bias?
Hi,
I just got my first NovaSeq-produced RNASeq dataset and the obtained lib sizes are quite uneven. The attached file shows the lib sizes. If...Last edited by David [R]; 02-19-2019, 01:16 AM. -
anyone with experience of using new RNA lib prep kit for FFPE samples on Novseq
Due to high capacity of Novaseq machine, Illumina recommends that a new library kit be used for unique double indexing.
I used to run... -
very low coverage WGS on Novaseq 6000?
Is it possible to multiplex more than 384 samples on a Novaseq 6000 in order to obtain very low coverage WGS (0.5-2x)? -
Novaseq vs Miseq failure
Hi all,
This is the first time I'm trying to run a MIP (molecular inversion probe) library of ~800 genomic targets, dual index library... -
gDNA library prep kit for NovaSeq 6000?
Going to have a NovaSeq installed soon and wonder which library prep kits are most optimal for human gDNA? We are going to use the libraries for both...
![David [R]](data:image/svg+xml;base64,CgkJCTxzdmcgeG1sbnM9J2h0dHA6Ly93d3cudzMub3JnLzIwMDAvc3ZnJyB2aWV3Qm94PScwIDAgNTAgNTAnPgoJCQkJPHJlY3Qgd2lkdGg9JzEwMCUnIGhlaWdodD0nMTAwJScgZmlsbD0nI2RhNmNlMCcgLz4KCQkJCTx0ZXh0IHg9JzUwJScgeT0nNTAlJyBmaWxsPSd3aGl0ZScgZm9udC13ZWlnaHQ9J2JvbGQnIGZvbnQtZmFtaWx5PSdBcmlhbCcgZm9udC1zaXplPSczMDAlJyBkb21pbmFudC1iYXNlbGluZT0nY2VudHJhbCcgdGV4dC1hbmNob3I9J21pZGRsZSc+RDwvdGV4dD4KCQkJPC9zdmc+CgkJ "David [R]")
