Hello everyone,
I'm currently working with PacBio HiFi CCS reads data for the HDV virus, and I've been using tools like pacbiominimap2 (PBMM2)...
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Having poor coverage depth using Pacbio hifi ccs reads for HDV viral Genome.
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Pacific Biosciences: Spotlight on the Companies Shaping Genetic Research
Established in 2004, Pacific Biosciences (PacBio) set out to create innovative tools that would elevate our understanding of genomics. Over the years, they have grown into global leaders in the field, known for their revolutionary HiFi sequencing—a high-accuracy, long-read sequencing solution.
Their journey began with the PacBio RS, their first commercial release, which employed their Single Molecule, Real-Time (SMRT) sequencing technology. This marked the beginning... -
Onso from Pacific Biosciences: Latest Sequencing Technologies—Part 4
Onso was the second sequencer Pacific Biosciences (PacBio) revealed during this year’s American Society of Human Genetics meeting (read about the first sequencer here). This benchtop sequencer uses sequencing by binding (SBB), a method unlike any other instrument on the market. SBB incorporates native nucleotides, has reduced molecular scarring, and reportedly results in significantly higher accuracy base calls than traditional short-read sequencers. The following sections highlight the important...
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How to analyse base mods on personal computer
I would like an opinion on how best to analyse pacbio data for base modifications when there is no access to a sequencing facility running an established... -
PacBio Metagenomics Webinar
Register to Attend: https://programs.pacificbiosciences....1-02-14/42m9qg
An ongoing challenge in microbiome research is achieving... -
Genome assembly and annotation tips sharing
De novo genome assembly has become an affordable and achievable method to solve biological problems. Following the recent Genome of Animal and Plants... -
Analyzing PacBio sequel data and SRA
I am new to NCBI's SRA and pacbio sequencing data analysis. I am trying to download pacbio sequence data from SRA and my goal is get CCS (Consensus) reads... -
PacBio software vcf and consensus fasta do not agree
I'm using PacBio's "variantCaller" tool (v6) to call variants on some older RSII subread data I aligned with blasr. The reference is 14845... -
PacBio software vcf and consensus fasta do not agree
I'm using your "variantCaller" tool (v6) to call variants on some older RSII subread data I aligned with blasr. The reference is 14845 bp.... -
Pangenomes Webinar: The Advantages of Sequencing Multiple Individuals
Date: Wednesday, September 16, 2020
Time: 10:00 a.m. PDT, 1:00 p.m. EDT, 6:00 p.m. BST
Unless you have an identical twin, no other... -
Webinar: Sequencing the Gut Microbiome with Highly Accurate Long Reads
Learn how researchers are using PacBio HiFi sequencing to overcome common challenges in microbiome research, including ambiguous taxonomic assignments... -
Webinar: How Long-Read Sequencing Improves Access to Genetic Information
Wondering if long-read sequencing is the right approach for your research? Join us on February 27 as we provide an introduction to our technology and... -
PacBio Webinar: How Long-Read Sequencing Improves Access to Genetic Information
Wondering if long-read sequencing is the right approach for your research? Join us on February 27 as we provide an introduction to our technology and... -
Webinar: How Long-Read Sequencing Improves Access to Genetic Information
Wondering if long-read sequencing is the right approach for your research? Join us on February 27 as we provide an introduction to our technology and... -
PacBio maintenance plan
Hi there,
We were recently awarded a grant to purchase a PacBio Sequel 2 NGS. It won't see much use for some time, but our organization...
