Hello there,
we consider MBD-seq cause the costs and overall CpG coverage. But I have limited understanding regarding number of reads from Illumina....
82 results in 0.0034 seconds.
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MBD-seq single-end or paired-end - number of reads
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Artemis paired end illumina short read mapping won't display any "duplicate" reads
Hi! I'm working with Illumina paired end short reads fastq mapping using Artemis for a course I'm taking. While I'm able to get all SAM/BAM files without... -
Is there a paired end joiner which writes also the reads before merging?
Hi, I have a weird question. But I'm looking for a software which not just merge the paired reads but also writes them in a new file.
When... -
Basequality Dropoff
Hello people,
first time poster and also kind of first time sequencer.
I just started my PhD which involves a lot of NGS on a MiSeq... -
RNA-seq in the NovaSeq
The smallest S1 kit is 100-cycles. With RNA-seq this results in a choice of single-end (100bp) or paired-end (2x50).
For the detection... -
STACKS and PE Illumina Data (ddRAD)
I have a dataset from a ddRAD library (Illumina HiSeq4K, 150PE). I'm playing around with STACKS, and noticed that it has some limitations when dealing... -
Paired end fastq with very very different sizes between samples
Hello , I have received some paired end samples to analyze.
I'm new at all so, i don't know if it is normal but the R1 and R2 fastq for the same... -
Bowtie2 paired end alignments do not match
Hi all,
Maybe someone already encoutered this issue... I am trying to use bowtie2 to map some paired-end reads against a set of assembled... -
Retrieving single and reads from paired end read sequencing run
Hi everyone,
As a first single cell RNAseq experiment, we would like to compare between single end and paired end sequencing. So at the beggining... -
Poor alignment rate by bowtie2 for exome sequence data
Hi
I am aligning the paired exome sequencing data, that originated from
Sorted raw data bam file -->
Converted to fastq... -
RNA-Seq quantitation for human, paired end vs single end, strand specific or not?
Is there any consensus of opinion on the best RNA-Seq approach for quantitation of alternative splicing on well annotated genomes (e.g. human)? We don’t... -
Paired end in Ion Torrent
Hi you all!
I need your help... I'm trying to search for a translocation using ion torrent sequencing (i know the genes involved in the... -
DEXSeq error with paired-end data
Hi,
I'm trying to run rhe python script for counting the reads for a dexseq analysis.
But I keep getting a strange error I can't... -
SPAdes: with different read length
Hi members,
I'm facing something weird. I know not all reads in the fastq files can have same length.
But in my trimmed data, read... -
output for sequencing of reverse strand
Hi Members,
I've lost, yet again with direction, and sequencing. I'm working on 16s data. Paired end, Illumina reads. And since then I am...Last edited by bio_informatics; 06-09-2015, 08:26 AM.
