8 results in 0.0055 seconds.
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Paired-ends data aligning preferentially to specific strands
I have some PE bs-seq data which is aligning very strangely: the left reads are aligning only to the - strand and the right reads only to the + strand.... -
pooled shRNA screen and analysis by Illumina HiSeq
Hi,
We want to do pooled shRNA screens using the TRC library. The goal is to use NGS to look at shRNA representation before and after selaction.... -
Why HTseq warning of unfound mate pairs?
Dear all
I am using htseq-count tool to summarize gene counts from bam files generated by tophat (v 2.03) based on bowtie2. I've used this pipeline... -
SGA preprocess error, read ids
SGA preprocess is giving me the following error:
Warning: Pair IDs do not match (expected format /1,/2 or /A,/B)
Read1 ID: HWUSI-EAS558R_0015:7:26:4067:7956#0/1... -
Denovo Hybrid Assembly using 454/illumina
Hi,
I have managed to get a reasonable assembly of a bacterial genome using newbler based on 454 NG sequence data.
I have... -
SageSigh
Hi. I am GaryM from Sage Science. Glad to be a part of the SEQanswers community. In addition to AGBT, I am wondering what other sequencing-centric meetings... -
Paired end generation
When are the paired ends generated? During the Cluster generation step or during the sequencing step?
The fact that you need twice as... -
cufflinks without paired reads
I'm just getting my feet wet with cufflinks.
Is there an option for specifying that I do not have paired reads?
Thanks,...
